TY - JOUR

T1 - The use of a “horizontal centrifugation protocol” to prepare autologous platelet-rich fibrin membranes for corneal reconstruction surgery in dogs with complicated corneal ulcerations

T2 - A case series

AU - Baadsgaard Bruun, Louise

AU - Hardon, Tommy A. Waskiw

AU - Krogh, Anne K. Havnsøe

AU - Qvortup, Klaus

AU - Heegaard, Steffen

AU - Kristensen, Annemarie Thuri

AU - Henriksen, Michala de Linde

N1 - Publisher Copyright: © 2023 The Authors. Veterinary Ophthalmology published by Wiley Periodicals LLC on behalf of American College of Veterinary Ophthalmologists.

PY - 2024

Y1 - 2024

N2 - Objective: The purpose of this case series was to describe the effect of autologous PRF membrane for corneal reconstruction surgery in dogs. PRF membranes made from two healthy dogs unrelated to the current case series were used for PRF histologic analyses. Animals: Seven dogs with complicated corneal ulcerations. Procedure: A complete ophthalmic examination, hematology, and fibrinogen analysis were performed pre-surgery. A PRF clot was made from autologous blood in a serum tube after centrifugation in a horizontal Bio-PRF® Centrifuge at 700 × g for 8 min. The PRF clot was processed in a PRF-Box® into a PRF membrane. The PRF membrane was sutured to the corneal ulcer bed. Each dog had a follow-up at days 5–7, 12–14, and 30–40 post-surgery. A final long-term follow-up was performed as well. Results: A positive outcome with healing and a “good” quality PRF membrane was seen in six out of seven dogs. One dog had a fibrinogen level below normal range and the PRF membrane was of “poor” quality. This dog developed a descemetocele 13 days post-surgery and needed rescue surgery. Mean healing time for all dogs was 9 ± 5.5 days. Minimal scarring, corneal pigmentation, and vascularization were observed at the final long-term follow-up 288 ± 44 days post-surgery. Conclusion: PRF membrane was successful as graft material for corneal ulceration reconstruction surgery. Low fibrinogen appeared to have negative effect on the quality of the PRF membrane, showing the importance for the surgeon to evaluate the quality of the PRF membrane prior to surgery.

AB - Objective: The purpose of this case series was to describe the effect of autologous PRF membrane for corneal reconstruction surgery in dogs. PRF membranes made from two healthy dogs unrelated to the current case series were used for PRF histologic analyses. Animals: Seven dogs with complicated corneal ulcerations. Procedure: A complete ophthalmic examination, hematology, and fibrinogen analysis were performed pre-surgery. A PRF clot was made from autologous blood in a serum tube after centrifugation in a horizontal Bio-PRF® Centrifuge at 700 × g for 8 min. The PRF clot was processed in a PRF-Box® into a PRF membrane. The PRF membrane was sutured to the corneal ulcer bed. Each dog had a follow-up at days 5–7, 12–14, and 30–40 post-surgery. A final long-term follow-up was performed as well. Results: A positive outcome with healing and a “good” quality PRF membrane was seen in six out of seven dogs. One dog had a fibrinogen level below normal range and the PRF membrane was of “poor” quality. This dog developed a descemetocele 13 days post-surgery and needed rescue surgery. Mean healing time for all dogs was 9 ± 5.5 days. Minimal scarring, corneal pigmentation, and vascularization were observed at the final long-term follow-up 288 ± 44 days post-surgery. Conclusion: PRF membrane was successful as graft material for corneal ulceration reconstruction surgery. Low fibrinogen appeared to have negative effect on the quality of the PRF membrane, showing the importance for the surgeon to evaluate the quality of the PRF membrane prior to surgery.

KW - clinical study

KW - cornea

KW - graft material

KW - infected corneal ulceration

KW - methodology

KW - pedicle conjunctival graft

U2 - 10.1111/vop.13148

DO - 10.1111/vop.13148

M3 - Journal article

C2 - 37721981

AN - SCOPUS:85171382647

JO - Veterinary Ophthalmology

JF - Veterinary Ophthalmology

SN - 1463-5216

ER -