Cell therapy
- Cell therapy
- Selection of the best possible cell source for cell therapy in orthopaedic disorders – tissue formation potential and response to an inflammatory micro-environment. A comparative study between stem cells and adult chondrocytes and osteocytes (3-year FTP Research Council project)
Selection of the best possible cell source for cell therapy in orthopaedic disorders – tissue formation potential and response to an inflammatory micro-environment.
A comparative study between stem cells and adult chondrocytes and osteocytes (3-year FTP Research Council project)
Aim: To determine the best choice of cells for orthopaedic disorders based on their performance in the microenvironment of tissue lesions, thus enabling clinicians to make research-based choices when selecting cells.
Short description: The body has an amazing ability to repair tissue, but sometimes it fails. Bone and cartilage defects are particularly problematic, and cell-based treatments are increasingly important for orthopaedic disorders in human and veterinary medicine. Current and potential cell sources include mesenchymal stem cells, mature tissue cells, and the promising induced pluripotent stem cells (iPS). Choice of cell type ought to be based on the cells’ ability to generate tissue, but also how they perform in an injury environment, where they will be affected by inflammatory factors and antiinflammatory medications. Yet, cell choice is currently primarily influenced by commercial interests, convenience of sampling, traditions, and cost.
So which cell type should we ideally choose for cell therapy in orthopaedic disorders?
And does ‘one cell fit all’ or can we tailor our cell selection for specific tissues or disorders?
Project hypotheses:
1 – Equine iPS have more chondrogenic and osteogenic potential than MSC from bone marrow, adipose tissue, and peripheral blood
2 – Transduction / transfection of progenitor cells increases their potential for formation of cartilage and bone
3 – Formation of cartilage and bone is not affected equally by an inflammatory microenvironment in different progenitor cells
4 – Differentiation status (mature, partial, or naïve) affect formation of cartilage and bone in an inflammatory microenvironment
5 – Anti-inflammatory drugs have a negative effect on formation of cartilage and bone
Principal investigator: Lise C. Berg
Collaborators: Preben D. Thomsen, Louise Bundgaard, Jasmin Bagge
Time frame: September 2014 - December 2017
Two review papers published in Canadian Veterinary Journal:
Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine. Koch TG, Berg LC, Betts DH. Can Vet J. 2009 Feb;50(2):155-65. PubMed
Concepts for the clinical use of stem cells in equine medicine. Koch TG, Berg LC, Betts DH. Can Vet J. 2008 Oct;49(10):1009-17. PubMed
Two Danish language review papers:
Stamceller - del 1: Hvad er de, og hvad kan de bruges til? Dansk Veterinærtidsskrift. 2008 Juli 14:8-11.
Stamceller - del 2: Led- og senebehandling nu og i fremtiden. Dansk Veterinærtidsskrift. 2008 Aug 15/16:10-14.