Protection against Chlamydia trachomatis infection and upper genital tract pathological changes by vaccine-promoted neutralizing antibodies directed to the VD4 of the major outer membrane protein
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- Protection against Chlamydia trachomatis infection
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The VD4 region from the Chlamydia trachomatis major outer membrane protein contains important neutralizing
B-cell epitopes of relevance for antibody-mediated protection against genital tract infection. We developed a multivalent
vaccine construct based on VD4s and their surrounding constant segments from serovars D, E, and
F. Adjuvanted with cationic liposomes, this construct promoted strong immune responses to serovar-specific
epitopes, the conserved LNPTIAG epitope and neutralized serovars D, E, and F. Vaccinated mice were protected
against challenge, with protection defined as reduced bacterial numbers in vagina and prevention of pathological
changes in the upper genital tract. Adoptive transfer of serumand T-cell depletion experiments demonstrated a dominant
role for antibodies and CD4+ T cells in the protective immune response. Integrating a multivalent VD4 construct
into the sequence of the major outer membrane protein resulted in a protective and broadly neutralizing
vaccine. Our findings emphasize the important role of antibodies in protection against Chlamydia trachomatis.
Original language | English |
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Journal | Journal of Infectious Diseases |
Volume | 212 |
Issue number | 6 |
Pages (from-to) | 978-989 |
Number of pages | 12 |
ISSN | 0022-1899 |
DOIs | |
Publication status | Published - 15 Sep 2015 |
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