Automated Spectrophotometric Assays for the Measurement of Ammonia and Bicarbonate in Saliva of Horses: Analytical Validation and Changes in Equine Gastric Ulcer Syndrome (EGUS)
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Automated Spectrophotometric Assays for the Measurement of Ammonia and Bicarbonate in Saliva of Horses : Analytical Validation and Changes in Equine Gastric Ulcer Syndrome (EGUS). / Muñoz-Prieto, Alberto; Llamas-Amor, Eva; Contreras-Aguilar, María Dolores; Ayala, Ignacio; Martín Cuervo, María; Cerón, José Joaquín; Hansen, Sanni.
In: Metabolites, Vol. 14, No. 3, 147, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Automated Spectrophotometric Assays for the Measurement of Ammonia and Bicarbonate in Saliva of Horses
T2 - Analytical Validation and Changes in Equine Gastric Ulcer Syndrome (EGUS)
AU - Muñoz-Prieto, Alberto
AU - Llamas-Amor, Eva
AU - Contreras-Aguilar, María Dolores
AU - Ayala, Ignacio
AU - Martín Cuervo, María
AU - Cerón, José Joaquín
AU - Hansen, Sanni
N1 - Publisher Copyright: © 2024 by the authors.
PY - 2024
Y1 - 2024
N2 - Ammonia (NH3) and bicarbonate (HCO3) have been related to gastric ulcers in humans. Ammonia is considered a possible cause of gastric ulcers, whereas bicarbonate has a protective function. The presence of ulcers in the stomach of horses is defined as Equine Gastric Ulcer Syndrome (EGUS), which is a frequent disease in this species, and it has been associated with changes in saliva composition, such as in analytes related to inflammation, immune system and oxidative stress. The objectives of this study were (1) to perform an analytical validation of two automated spectrophotometric assays, one for ammonia and one for bicarbonate, in the horses’ saliva and (2) to evaluate their possible variations with EGUS. Analytical validation of the automated assays for ammonia and bicarbonate in the saliva of horses showed that both assays were precise and accurate. In addition, significantly higher values of ammonia and lower values of bicarbonate were found in the saliva of horses with EGUS compared to healthy horses. It can be concluded that ammonia and bicarbonate can be measured in the saliva of horses and that ammonia increases and bicarbonate decreases in this sample type could be related to the presence of EGUS in this species.
AB - Ammonia (NH3) and bicarbonate (HCO3) have been related to gastric ulcers in humans. Ammonia is considered a possible cause of gastric ulcers, whereas bicarbonate has a protective function. The presence of ulcers in the stomach of horses is defined as Equine Gastric Ulcer Syndrome (EGUS), which is a frequent disease in this species, and it has been associated with changes in saliva composition, such as in analytes related to inflammation, immune system and oxidative stress. The objectives of this study were (1) to perform an analytical validation of two automated spectrophotometric assays, one for ammonia and one for bicarbonate, in the horses’ saliva and (2) to evaluate their possible variations with EGUS. Analytical validation of the automated assays for ammonia and bicarbonate in the saliva of horses showed that both assays were precise and accurate. In addition, significantly higher values of ammonia and lower values of bicarbonate were found in the saliva of horses with EGUS compared to healthy horses. It can be concluded that ammonia and bicarbonate can be measured in the saliva of horses and that ammonia increases and bicarbonate decreases in this sample type could be related to the presence of EGUS in this species.
KW - ammonia
KW - bicarbonate
KW - EGUS
KW - horses
KW - saliva
U2 - 10.3390/metabo14030147
DO - 10.3390/metabo14030147
M3 - Journal article
C2 - 38535307
AN - SCOPUS:85188943885
VL - 14
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 3
M1 - 147
ER -
ID: 389411854