Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1

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Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1. / Staunstrup, Nicklas Heine; Stenderup, Karin; Mortensen, Sidsel; Primo, Maria Nascimento; Rosada, Cecilia; Steiniche, Torben; Liu, Ying; Li, Rong; Schmidt, Mette; Purup, Stig; Dagnæs-Hansen, Frederik; Schrøder, Lisbeth Dahl; Svensson, Lars; Petersen, Thomas Kongstad; Callesen, Henrik; Bolund, Lars; Mikkelsen, Jacob Giehm.

In: Disease Models & Mechanisms, Vol. 10, No. 7, 2017, p. 869-880.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Staunstrup, NH, Stenderup, K, Mortensen, S, Primo, MN, Rosada, C, Steiniche, T, Liu, Y, Li, R, Schmidt, M, Purup, S, Dagnæs-Hansen, F, Schrøder, LD, Svensson, L, Petersen, TK, Callesen, H, Bolund, L & Mikkelsen, JG 2017, 'Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1', Disease Models & Mechanisms, vol. 10, no. 7, pp. 869-880. https://doi.org/10.1242/dmm.028662

APA

Staunstrup, N. H., Stenderup, K., Mortensen, S., Primo, M. N., Rosada, C., Steiniche, T., Liu, Y., Li, R., Schmidt, M., Purup, S., Dagnæs-Hansen, F., Schrøder, L. D., Svensson, L., Petersen, T. K., Callesen, H., Bolund, L., & Mikkelsen, J. G. (2017). Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1. Disease Models & Mechanisms, 10(7), 869-880. https://doi.org/10.1242/dmm.028662

Vancouver

Staunstrup NH, Stenderup K, Mortensen S, Primo MN, Rosada C, Steiniche T et al. Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1. Disease Models & Mechanisms. 2017;10(7):869-880. https://doi.org/10.1242/dmm.028662

Author

Staunstrup, Nicklas Heine ; Stenderup, Karin ; Mortensen, Sidsel ; Primo, Maria Nascimento ; Rosada, Cecilia ; Steiniche, Torben ; Liu, Ying ; Li, Rong ; Schmidt, Mette ; Purup, Stig ; Dagnæs-Hansen, Frederik ; Schrøder, Lisbeth Dahl ; Svensson, Lars ; Petersen, Thomas Kongstad ; Callesen, Henrik ; Bolund, Lars ; Mikkelsen, Jacob Giehm. / Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1. In: Disease Models & Mechanisms. 2017 ; Vol. 10, No. 7. pp. 869-880.

Bibtex

@article{28cc2091d16a405b97aee6d0d7a7725a,
title = "Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1",
abstract = "Psoriasis is a complex human-specific disease characterized by perturbed keratinocyte proliferation and a pro-inflammatory environment in the skin. Porcine skin architecture and immunity are very similar to that in humans, rendering the pig a suitable animal model for studying the biology and treatment of psoriasis. Expression of integrins, which is normally confined to the basal layer of the epidermis, is maintained in suprabasal keratinocytes in psoriatic skin, modulating proliferation and differentiation as well as leukocyte infiltration. Here, we generated minipigs co-expressing integrins α2 and β1 in suprabasal epidermal layers. Integrin-transgenic minipigs born into the project displayed skin phenotypes that correlated with the number of inserted transgenes. Molecular analyses were in good concordance with histological observations of psoriatic hallmarks, including hypogranulosis and T-lymphocyte infiltration. These findings mark the first creation of minipigs with a psoriasiform phenotype resembling human psoriasis and demonstrate that integrin signaling plays a key role in psoriasis pathology.",
keywords = "DNA transposition, Integrin, Pig cloning, Psoriasis, Skin inflammation, Sleeping Beauty",
author = "Staunstrup, {Nicklas Heine} and Karin Stenderup and Sidsel Mortensen and Primo, {Maria Nascimento} and Cecilia Rosada and Torben Steiniche and Ying Liu and Rong Li and Mette Schmidt and Stig Purup and Frederik Dagn{\ae}s-Hansen and Schr{\o}der, {Lisbeth Dahl} and Lars Svensson and Petersen, {Thomas Kongstad} and Henrik Callesen and Lars Bolund and Mikkelsen, {Jacob Giehm}",
year = "2017",
doi = "10.1242/dmm.028662",
language = "English",
volume = "10",
pages = "869--880",
journal = "Disease Models & Mechanisms",
issn = "1754-8403",
publisher = "company of biologists",
number = "7",

}

RIS

TY - JOUR

T1 - Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1

AU - Staunstrup, Nicklas Heine

AU - Stenderup, Karin

AU - Mortensen, Sidsel

AU - Primo, Maria Nascimento

AU - Rosada, Cecilia

AU - Steiniche, Torben

AU - Liu, Ying

AU - Li, Rong

AU - Schmidt, Mette

AU - Purup, Stig

AU - Dagnæs-Hansen, Frederik

AU - Schrøder, Lisbeth Dahl

AU - Svensson, Lars

AU - Petersen, Thomas Kongstad

AU - Callesen, Henrik

AU - Bolund, Lars

AU - Mikkelsen, Jacob Giehm

PY - 2017

Y1 - 2017

N2 - Psoriasis is a complex human-specific disease characterized by perturbed keratinocyte proliferation and a pro-inflammatory environment in the skin. Porcine skin architecture and immunity are very similar to that in humans, rendering the pig a suitable animal model for studying the biology and treatment of psoriasis. Expression of integrins, which is normally confined to the basal layer of the epidermis, is maintained in suprabasal keratinocytes in psoriatic skin, modulating proliferation and differentiation as well as leukocyte infiltration. Here, we generated minipigs co-expressing integrins α2 and β1 in suprabasal epidermal layers. Integrin-transgenic minipigs born into the project displayed skin phenotypes that correlated with the number of inserted transgenes. Molecular analyses were in good concordance with histological observations of psoriatic hallmarks, including hypogranulosis and T-lymphocyte infiltration. These findings mark the first creation of minipigs with a psoriasiform phenotype resembling human psoriasis and demonstrate that integrin signaling plays a key role in psoriasis pathology.

AB - Psoriasis is a complex human-specific disease characterized by perturbed keratinocyte proliferation and a pro-inflammatory environment in the skin. Porcine skin architecture and immunity are very similar to that in humans, rendering the pig a suitable animal model for studying the biology and treatment of psoriasis. Expression of integrins, which is normally confined to the basal layer of the epidermis, is maintained in suprabasal keratinocytes in psoriatic skin, modulating proliferation and differentiation as well as leukocyte infiltration. Here, we generated minipigs co-expressing integrins α2 and β1 in suprabasal epidermal layers. Integrin-transgenic minipigs born into the project displayed skin phenotypes that correlated with the number of inserted transgenes. Molecular analyses were in good concordance with histological observations of psoriatic hallmarks, including hypogranulosis and T-lymphocyte infiltration. These findings mark the first creation of minipigs with a psoriasiform phenotype resembling human psoriasis and demonstrate that integrin signaling plays a key role in psoriasis pathology.

KW - DNA transposition

KW - Integrin

KW - Pig cloning

KW - Psoriasis

KW - Skin inflammation

KW - Sleeping Beauty

U2 - 10.1242/dmm.028662

DO - 10.1242/dmm.028662

M3 - Journal article

C2 - 28679670

AN - SCOPUS:85027038285

VL - 10

SP - 869

EP - 880

JO - Disease Models & Mechanisms

JF - Disease Models & Mechanisms

SN - 1754-8403

IS - 7

ER -

ID: 184391478