Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH

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Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH. / Andrade, Evelyn Rabelo; Hyttel, Poul; Landim-Alvarenga, Fernanda Da Cruz ; Silva, José Roberto Viana; Alfieri, Amauri Alcindo; Seneda, Marcelo Marcondes; Figueiredo, José Ricardo; Toniolli, Ricardo.

In: Veterinary Medicine International, Vol. 2011, 2011.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andrade, ER, Hyttel, P, Landim-Alvarenga, FDC, Silva, JRV, Alfieri, AA, Seneda, MM, Figueiredo, JR & Toniolli, R 2011, 'Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH', Veterinary Medicine International, vol. 2011. https://doi.org/10.4061/2011/670987

APA

Andrade, E. R., Hyttel, P., Landim-Alvarenga, F. D. C., Silva, J. R. V., Alfieri, A. A., Seneda, M. M., Figueiredo, J. R., & Toniolli, R. (2011). Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH. Veterinary Medicine International, 2011. https://doi.org/10.4061/2011/670987

Vancouver

Andrade ER, Hyttel P, Landim-Alvarenga FDC, Silva JRV, Alfieri AA, Seneda MM et al. Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH. Veterinary Medicine International. 2011;2011. https://doi.org/10.4061/2011/670987

Author

Andrade, Evelyn Rabelo ; Hyttel, Poul ; Landim-Alvarenga, Fernanda Da Cruz ; Silva, José Roberto Viana ; Alfieri, Amauri Alcindo ; Seneda, Marcelo Marcondes ; Figueiredo, José Ricardo ; Toniolli, Ricardo. / Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH. In: Veterinary Medicine International. 2011 ; Vol. 2011.

Bibtex

@article{a61311f7f7254764ba821a395cd08411,
title = "Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH",
abstract = "The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6¿d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.",
author = "Andrade, {Evelyn Rabelo} and Poul Hyttel and Landim-Alvarenga, {Fernanda Da Cruz} and Silva, {Jos{\'e} Roberto Viana} and Alfieri, {Amauri Alcindo} and Seneda, {Marcelo Marcondes} and Figueiredo, {Jos{\'e} Ricardo} and Ricardo Toniolli",
year = "2011",
doi = "10.4061/2011/670987",
language = "English",
volume = "2011",
journal = "Veterinary Medicine International",
issn = "2042-0048",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH

AU - Andrade, Evelyn Rabelo

AU - Hyttel, Poul

AU - Landim-Alvarenga, Fernanda Da Cruz

AU - Silva, José Roberto Viana

AU - Alfieri, Amauri Alcindo

AU - Seneda, Marcelo Marcondes

AU - Figueiredo, José Ricardo

AU - Toniolli, Ricardo

PY - 2011

Y1 - 2011

N2 - The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6¿d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

AB - The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6¿d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

U2 - 10.4061/2011/670987

DO - 10.4061/2011/670987

M3 - Journal article

VL - 2011

JO - Veterinary Medicine International

JF - Veterinary Medicine International

SN - 2042-0048

ER -

ID: 35084728