Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis
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Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis. / Anderson, James R.; Jacobsen, Stine; Walters, Marie; Bundgaard, Louise; Diendorfer, Andreas; Hackl, Matthias; Clarke, Emily J.; James, Victoria; Peffers, Mandy J.
In: Frontiers in Veterinary Science, Vol. 9, 901269, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis
AU - Anderson, James R.
AU - Jacobsen, Stine
AU - Walters, Marie
AU - Bundgaard, Louise
AU - Diendorfer, Andreas
AU - Hackl, Matthias
AU - Clarke, Emily J.
AU - James, Victoria
AU - Peffers, Mandy J.
N1 - Publisher Copyright: Copyright © 2022 Anderson, Jacobsen, Walters, Bundgaard, Diendorfer, Hackl, Clarke, James and Peffers.
PY - 2022
Y1 - 2022
N2 - Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that the small non-coding RNA expression pattern in synovial fluid and plasma would change during progression of experimental osteoarthritis. In this study, we conducted small RNA sequencing to provide a comprehensive overview of the temporal expression profiles of small non-coding transcripts carried by extracellular vesicles derived from plasma and synovial fluid for the first time in a posttraumatic model of equine osteoarthritis. Additionally, we characterised synovial fluid and plasma-derived extracellular vesicles with respect to quantity, size, and surface markers. The different temporal expressions of seven microRNAs in plasma and synovial fluid-derived extracellular vesicles, eca-miR-451, eca-miR-25, eca-miR-215, eca-miR-92a, eca-miR-let-7c, eca-miR-486-5p, and eca-miR-23a, and four snoRNAs, U3, snord15, snord46, and snord58, represent potential biomarkers for early osteoarthritis. Bioinformatics analysis of the differentially expressed microRNAs in synovial fluid highlighted that in early osteoarthritis these related to the inhibition of cell cycle, cell cycle progression, DNA damage and cell proliferation as well as increased cell viability and differentiation of stem cells. Plasma and synovial fluid-derived extracellular vesicle small non-coding signatures have been established for the first time in a temporal model of osteoarthritis. These could serve as novel biomarkers for evaluation of osteoarthritis progression or act as potential therapeutic targets.
AB - Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that the small non-coding RNA expression pattern in synovial fluid and plasma would change during progression of experimental osteoarthritis. In this study, we conducted small RNA sequencing to provide a comprehensive overview of the temporal expression profiles of small non-coding transcripts carried by extracellular vesicles derived from plasma and synovial fluid for the first time in a posttraumatic model of equine osteoarthritis. Additionally, we characterised synovial fluid and plasma-derived extracellular vesicles with respect to quantity, size, and surface markers. The different temporal expressions of seven microRNAs in plasma and synovial fluid-derived extracellular vesicles, eca-miR-451, eca-miR-25, eca-miR-215, eca-miR-92a, eca-miR-let-7c, eca-miR-486-5p, and eca-miR-23a, and four snoRNAs, U3, snord15, snord46, and snord58, represent potential biomarkers for early osteoarthritis. Bioinformatics analysis of the differentially expressed microRNAs in synovial fluid highlighted that in early osteoarthritis these related to the inhibition of cell cycle, cell cycle progression, DNA damage and cell proliferation as well as increased cell viability and differentiation of stem cells. Plasma and synovial fluid-derived extracellular vesicle small non-coding signatures have been established for the first time in a temporal model of osteoarthritis. These could serve as novel biomarkers for evaluation of osteoarthritis progression or act as potential therapeutic targets.
KW - extracellular vesicles
KW - osteoarthritis
KW - plasma
KW - small non-coding RNA
KW - synovial fluid
U2 - 10.3389/fvets.2022.901269
DO - 10.3389/fvets.2022.901269
M3 - Journal article
C2 - 36003409
AN - SCOPUS:85136463674
VL - 9
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
SN - 2297-1769
M1 - 901269
ER -
ID: 319166033