Evaluation of the in vitro effects of local anesthetics on equine chondrocytes and fibroblast-like synoviocytes
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Evaluation of the in vitro effects of local anesthetics on equine chondrocytes and fibroblast-like synoviocytes. / Adler, Ditte M.T.; Frellesen, Jeppe F.; Karlsen, Christoffer V.; Jensen, Line D.; Dahm, Anne S.Q.; Berg, Lise C.
In: American Journal of Veterinary Research, Vol. 82, No. 6, 2021, p. 478-486.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Evaluation of the in vitro effects of local anesthetics on equine chondrocytes and fibroblast-like synoviocytes
AU - Adler, Ditte M.T.
AU - Frellesen, Jeppe F.
AU - Karlsen, Christoffer V.
AU - Jensen, Line D.
AU - Dahm, Anne S.Q.
AU - Berg, Lise C.
N1 - Publisher Copyright: © 2021, American Veterinary Medical Association. All rights reserved.
PY - 2021
Y1 - 2021
N2 - OBJECTIVE To investigate the in vitro effects of clinically relevant concentrations of the local anesthetics (LAs) bupivacaine, lidocaine, lidocaine with preservative (LP), mepivacaine, and ropivacaine on equine chondrocyte and fibroblast-like synoviocyte (FLS) viability. SAMPLES Chondrocytes and FLSs of the metacarpophalangeal joints of 4 healthy adult horses. PROCEDURES Viability of chondrocytes and FLSs was determined with 3 assays: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate de-hydrogenase (LDH), and trypan blue (TB) exclusion (only FLS). Viability was assessed after 30-and 60-minute exposures to 0.0625%, 0.125%, and 0.25% bupivacaine; 0.25%, 0.5%, and 1% lidocaine; 0.25%, 0.5%, and 1% LP; 0.25%, 0.5%, and 1% mepivacaine; and 0.125%, 0.25%, and 0.5% ropivacaine. RESULTS Viability of chondrocytes was significantly decreased with exposure to 0.25% bupivacaine, 1% lidocaine, 1% LP, 1% mepivacaine, and 0.25% ropiva-caine. Viability of FLSs was significantly decreased with exposure to 0.25% bupivacaine, 1% mepivacaine, 1% LP, and 0.5% ropivacaine. CONCLUSIONS AND CLINICAL RELEVANCE Clinically relevant concentrations of LAs had in vitro time-and concentra-tion-dependent cytotoxicity for chondrocytes and FLSs isolated from the metacarpophalangeal joints of healthy horses. Bupivacaine was more toxic to chondrocytes than lidocaine, mepivacaine, and ropivacaine, whereas bu-pivacaine, LP, mepivacaine, and ropivacaine were more toxic to FLSs than preservative-free lidocaine. Several LAs may negatively affect chondrocyte and FLS viability. (Am J Vet Res 2021;82:478–486).
AB - OBJECTIVE To investigate the in vitro effects of clinically relevant concentrations of the local anesthetics (LAs) bupivacaine, lidocaine, lidocaine with preservative (LP), mepivacaine, and ropivacaine on equine chondrocyte and fibroblast-like synoviocyte (FLS) viability. SAMPLES Chondrocytes and FLSs of the metacarpophalangeal joints of 4 healthy adult horses. PROCEDURES Viability of chondrocytes and FLSs was determined with 3 assays: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate de-hydrogenase (LDH), and trypan blue (TB) exclusion (only FLS). Viability was assessed after 30-and 60-minute exposures to 0.0625%, 0.125%, and 0.25% bupivacaine; 0.25%, 0.5%, and 1% lidocaine; 0.25%, 0.5%, and 1% LP; 0.25%, 0.5%, and 1% mepivacaine; and 0.125%, 0.25%, and 0.5% ropivacaine. RESULTS Viability of chondrocytes was significantly decreased with exposure to 0.25% bupivacaine, 1% lidocaine, 1% LP, 1% mepivacaine, and 0.25% ropiva-caine. Viability of FLSs was significantly decreased with exposure to 0.25% bupivacaine, 1% mepivacaine, 1% LP, and 0.5% ropivacaine. CONCLUSIONS AND CLINICAL RELEVANCE Clinically relevant concentrations of LAs had in vitro time-and concentra-tion-dependent cytotoxicity for chondrocytes and FLSs isolated from the metacarpophalangeal joints of healthy horses. Bupivacaine was more toxic to chondrocytes than lidocaine, mepivacaine, and ropivacaine, whereas bu-pivacaine, LP, mepivacaine, and ropivacaine were more toxic to FLSs than preservative-free lidocaine. Several LAs may negatively affect chondrocyte and FLS viability. (Am J Vet Res 2021;82:478–486).
U2 - 10.2460/ajvr.82.6.478
DO - 10.2460/ajvr.82.6.478
M3 - Journal article
C2 - 34032483
AN - SCOPUS:85106939926
VL - 82
SP - 478
EP - 486
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 6
ER -
ID: 273134510