TY - JOUR

T1 - Obesity-induced changes in gene expression in feline adipose and skeletal muscle tissue

AU - Stenberg, Kathrine

AU - Novotny, Guy W.

AU - Lutz, Thomas A.

AU - Mandrup-Poulsen, Thomas

AU - Bjørnvad, Charlotte Reinhard

N1 - Publisher Copyright: © 2023 The Authors. Journal of Animal Physiology and Animal Nutrition published by Wiley-VCH GmbH.

PY - 2023

Y1 - 2023

N2 - Indoor-confined cats are prone to developing obesity due to a sedentary life and an energy intake exceeding energy requirements. As in humans, feline obesity decreases insulin sensitivity and increases the risk of developing feline diabetes mellitus, but the pathophysiological mechanisms are currently poorly understood. Human obesity-related metabolic alterations seem to relate to changes in the expression of genes involved in glucose metabolism, insulin action and inflammation. The objective of the current study was to investigate changes in the expression of genes relating to obesity, glucose metabolism and inflammation in cats with non-experimentally induced obesity. Biopsies from the sartorius muscle and subcutaneous adipose tissue were obtained from 73 healthy, neutered, indoor-confined domestic shorthaired cats ranging from lean to obese. Quantification of obesity-related gene expression levels relative to glyceraldehyde-3-phosphate dehydrogenase was performed by quantitative real-time polymerase chain reaction. A negative association between obesity and adiponectin expression was observed in the adipose tissue (mean ± SD; normal weight, 27.30 × 10−3 ± 77.14 × 10−3; overweight, 2.89 × 10−3 ± 0.38 × 10−3 and obese, 2.93 × 10−3 ± 4.20 × 10−3, p < 0.05). In muscle, the expression of peroxisome proliferative activated receptor-γ2 and plasminogen activator inhibitor-1 was increased in the obese compared to the normal-weight cats, and resistin was increased in the normal-weight compared to the overweight cats. There were no detectable obesity-related changes in the messenger RNA levels of inflammatory cytokines. In conclusion, a possible obesity-related low-grade inflammation caused by increased expression of key proinflammatory regulators was not observed. This could imply that the development of feline obesity and ensuing insulin resistance may not be based on tissue-derived inflammation, but caused by several determining factors, many of which still need further investigation.

AB - Indoor-confined cats are prone to developing obesity due to a sedentary life and an energy intake exceeding energy requirements. As in humans, feline obesity decreases insulin sensitivity and increases the risk of developing feline diabetes mellitus, but the pathophysiological mechanisms are currently poorly understood. Human obesity-related metabolic alterations seem to relate to changes in the expression of genes involved in glucose metabolism, insulin action and inflammation. The objective of the current study was to investigate changes in the expression of genes relating to obesity, glucose metabolism and inflammation in cats with non-experimentally induced obesity. Biopsies from the sartorius muscle and subcutaneous adipose tissue were obtained from 73 healthy, neutered, indoor-confined domestic shorthaired cats ranging from lean to obese. Quantification of obesity-related gene expression levels relative to glyceraldehyde-3-phosphate dehydrogenase was performed by quantitative real-time polymerase chain reaction. A negative association between obesity and adiponectin expression was observed in the adipose tissue (mean ± SD; normal weight, 27.30 × 10−3 ± 77.14 × 10−3; overweight, 2.89 × 10−3 ± 0.38 × 10−3 and obese, 2.93 × 10−3 ± 4.20 × 10−3, p < 0.05). In muscle, the expression of peroxisome proliferative activated receptor-γ2 and plasminogen activator inhibitor-1 was increased in the obese compared to the normal-weight cats, and resistin was increased in the normal-weight compared to the overweight cats. There were no detectable obesity-related changes in the messenger RNA levels of inflammatory cytokines. In conclusion, a possible obesity-related low-grade inflammation caused by increased expression of key proinflammatory regulators was not observed. This could imply that the development of feline obesity and ensuing insulin resistance may not be based on tissue-derived inflammation, but caused by several determining factors, many of which still need further investigation.

KW - adipokines

KW - adiponectin

KW - cat

KW - inflammation

KW - leptin

KW - qPCR

U2 - 10.1111/jpn.13802

DO - 10.1111/jpn.13802

M3 - Journal article

C2 - 36591865

AN - SCOPUS:85145404362

VL - 107

SP - 1262

EP - 1278

JO - Journal of Animal Physiology and Animal Nutrition (Online)

JF - Journal of Animal Physiology and Animal Nutrition (Online)

SN - 1439-0396

IS - 5

ER -