Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
The objective of the study was to evaluate the gene expression of inflammatory cytokines (interleukin [IL]-1ß, IL-6, IL-8, IL-10, tumor necrosis factor [TNF]-a, IL-1 receptor antagonist [ra] and serum amyloid A (SAA) in endometrial tissue and circulating leukocytes in response to uterine inoculation of 105 colony forming units (CFU) Escherichia coli in mares. Before inoculation, mares were classified as resistant or susceptible to persistent endometritis based on their uterine inflammatory response to infusion of 109 killed spermatozoa and histological assessment of the endometrial quality. Endometrial biopsies were obtained 3, 12, 24 and 72 hours (h) after bacterial inoculation and blood samples were obtained during the 7 day period post bacterial inoculation. Expression levels of cytokines and SAA were determined by quantitative real-time reverse transcriptase PCR (qRT-PCR).
Originalsprog | Engelsk |
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Tidsskrift | B M C Veterinary Research |
Vol/bind | 8 |
Udgave nummer | 41 |
Antal sider | 14 |
ISSN | 1746-6148 |
DOI | |
Status | Udgivet - 2012 |
- Infectious endometritis, Susceptibility, Cytokines, Serum amyloid A
Forskningsområder
ID: 44663534