Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach
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Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach. / Muñoz-Prieto, Alberto; Contreras-Aguilar, Maria Dolores; Cerón, Jose Joaquín; Ayala, Ignacio; Martin-Cuervo, Maria; Gonzalez-Sanchez, Juan Carlos; Jacobsen, Stine; Kuleš, Josipa; Beletić, Anđelo; Rubić, Ivana; Mrljak, Vladimir; Tecles, Fernando; Hansen, Sanni.
I: Animals, Bind 12, Nr. 9, 1169, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach
AU - Muñoz-Prieto, Alberto
AU - Contreras-Aguilar, Maria Dolores
AU - Cerón, Jose Joaquín
AU - Ayala, Ignacio
AU - Martin-Cuervo, Maria
AU - Gonzalez-Sanchez, Juan Carlos
AU - Jacobsen, Stine
AU - Kuleš, Josipa
AU - Beletić, Anđelo
AU - Rubić, Ivana
AU - Mrljak, Vladimir
AU - Tecles, Fernando
AU - Hansen, Sanni
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022
Y1 - 2022
N2 - Changes in the salivary proteome in 12 horses with the two diseases included in equine gastric ulcer syndrome (EGUS), equine glandular gastric disease (EGGD) (n = 6) and equine squa-mous gastric disease (ESGD) (n = 6), were evaluated using a high-resolution LC-MS/MS analysis of TMT-labelled peptides and compared to 10 healthy control horses. Serum was also analysed for comparative purposes. The comparison between the horses with EGGD and controls showed significant changes in 10 salivary proteins, whereas 36 salivary proteins were differently abundant between ESGD and control groups. The most upregulated proteins in the case of EGGD were related to immune activation whereas, in horses with ESGD, the most significantly changed proteins were associated with squamous cell regulation and growth. Compared to serum, saliva showed a higher number of proteins with significant changes and a different pattern of changes. The proteins iden-tified in our study, in addition to providing new information about the pathophysiological mecha-nisms in these diseases, could have the potential to be novel biomarkers for the diagnosis or monitoring of EGGD and ESGD.
AB - Changes in the salivary proteome in 12 horses with the two diseases included in equine gastric ulcer syndrome (EGUS), equine glandular gastric disease (EGGD) (n = 6) and equine squa-mous gastric disease (ESGD) (n = 6), were evaluated using a high-resolution LC-MS/MS analysis of TMT-labelled peptides and compared to 10 healthy control horses. Serum was also analysed for comparative purposes. The comparison between the horses with EGGD and controls showed significant changes in 10 salivary proteins, whereas 36 salivary proteins were differently abundant between ESGD and control groups. The most upregulated proteins in the case of EGGD were related to immune activation whereas, in horses with ESGD, the most significantly changed proteins were associated with squamous cell regulation and growth. Compared to serum, saliva showed a higher number of proteins with significant changes and a different pattern of changes. The proteins iden-tified in our study, in addition to providing new information about the pathophysiological mecha-nisms in these diseases, could have the potential to be novel biomarkers for the diagnosis or monitoring of EGGD and ESGD.
KW - EGGD
KW - EGUS
KW - ESGD
KW - horse
KW - proteomics
KW - saliva
KW - serum
U2 - 10.3390/ani12091169
DO - 10.3390/ani12091169
M3 - Journal article
C2 - 35565595
AN - SCOPUS:85129432882
VL - 12
JO - Animals
JF - Animals
SN - 2076-2615
IS - 9
M1 - 1169
ER -
ID: 307747698