Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats
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Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats. / Søe, Niels Henrik; Jensen, Nina Vendel; Lundorff Jensen, Asger; Koch, Janne; Poulsen, Steen Seier; Pier, Gerald B; Johansen, Helle Krogh.
I: In Vivo, Bind 31, Nr. 1, 2017, s. 45-50.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats
AU - Søe, Niels Henrik
AU - Jensen, Nina Vendel
AU - Lundorff Jensen, Asger
AU - Koch, Janne
AU - Poulsen, Steen Seier
AU - Pier, Gerald B
AU - Johansen, Helle Krogh
N1 - Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
PY - 2017
Y1 - 2017
N2 - BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10(3) S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica(+)) but not ica(-) S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica(+) or ica(-) S. aureus, suggesting a lack of specificity in this antiserum.
AB - BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10(3) S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica(+)) but not ica(-) S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica(+) or ica(-) S. aureus, suggesting a lack of specificity in this antiserum.
U2 - 10.21873/invivo.11023
DO - 10.21873/invivo.11023
M3 - Journal article
C2 - 28064219
VL - 31
SP - 45
EP - 50
JO - In Vivo
JF - In Vivo
SN - 0258-851X
IS - 1
ER -
ID: 172266256