TY - JOUR

T1 - The Equine PeptideAtlas

T2 - a resource for developing proteomics-based veterinary research

AU - Bundgaard, Louise

AU - Jacobsen, Stine

AU - Sørensen, Mette Aamand

AU - Sun, Zhi

AU - Deutsch, Eric W.

AU - Moritz, Robert L.

AU - Bendixen, Emøke

PY - 2014

Y1 - 2014

N2 - Progress in MS-based methods for veterinary research and diagnostics is lagging behind compared to the human research, and proteome data of domestic animals is still not well represented in open source data repositories. This is particularly true for the equine species. Here wepresent a first Equine PeptideAtlas encompassing high-resolution tandem MS analyses of 51 samples representing a selection of equine tissues and body fluids from healthy and diseased animals. The raw data were processed through the Trans-Proteomic Pipeline to yield high quality identification of proteins and peptides. The current release comprises 24 131 distinct peptides representing 2636 canonical proteins observed at false discovery rates of 0.2% at the peptide level and 1.4% at the protein level. Data from the Equine PeptideAtlas are available for experimental planning, validation of new datasets, and as a proteomic data mining resource. The advantages of the Equine PeptideAtlas are demonstrated by examples of mining the contents for information on potential and well-known equine acute phase proteins, which have extensive general interest in the veterinary clinic. The extracted information will support further analyses, and emphasizes the value of the Equine PeptideAtlas as a resource for the design of targeted quantitative proteomic studies.

AB - Progress in MS-based methods for veterinary research and diagnostics is lagging behind compared to the human research, and proteome data of domestic animals is still not well represented in open source data repositories. This is particularly true for the equine species. Here wepresent a first Equine PeptideAtlas encompassing high-resolution tandem MS analyses of 51 samples representing a selection of equine tissues and body fluids from healthy and diseased animals. The raw data were processed through the Trans-Proteomic Pipeline to yield high quality identification of proteins and peptides. The current release comprises 24 131 distinct peptides representing 2636 canonical proteins observed at false discovery rates of 0.2% at the peptide level and 1.4% at the protein level. Data from the Equine PeptideAtlas are available for experimental planning, validation of new datasets, and as a proteomic data mining resource. The advantages of the Equine PeptideAtlas are demonstrated by examples of mining the contents for information on potential and well-known equine acute phase proteins, which have extensive general interest in the veterinary clinic. The extracted information will support further analyses, and emphasizes the value of the Equine PeptideAtlas as a resource for the design of targeted quantitative proteomic studies.

KW - Faculty of Health and Medical Sciences

KW - Horses

KW - Databases, Protein

KW - Proteomics

KW - Mass Spectrometry

KW - Veterinary medicine

U2 - 10.1002/pmic.201300398

DO - 10.1002/pmic.201300398

M3 - Journal article

C2 - 24436130

VL - 14

SP - 763

EP - 773

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 6

ER -