A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs. / Bøje, Sarah Henriette; Olsen, Anja Weinreich; Erneholm, Karin Susanne; Agerholm, Jørgen Steen; Jungersen, Gregers; Andersen, Peter; Follmann, Frank.

I: Immunology and Cell Biology, Bind 94, Nr. 2, 02.2016, s. 185-195.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Bøje, SH, Olsen, AW, Erneholm, KS, Agerholm, JS, Jungersen, G, Andersen, P & Follmann, F 2016, 'A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs', Immunology and Cell Biology, bind 94, nr. 2, s. 185-195. https://doi.org/10.1038/icb.2015.79

APA

Bøje, S. H., Olsen, A. W., Erneholm, K. S., Agerholm, J. S., Jungersen, G., Andersen, P., & Follmann, F. (2016). A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs. Immunology and Cell Biology, 94(2), 185-195. https://doi.org/10.1038/icb.2015.79

Vancouver

Bøje SH, Olsen AW, Erneholm KS, Agerholm JS, Jungersen G, Andersen P o.a. A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs. Immunology and Cell Biology. 2016 feb.;94(2):185-195. https://doi.org/10.1038/icb.2015.79

Author

Bøje, Sarah Henriette ; Olsen, Anja Weinreich ; Erneholm, Karin Susanne ; Agerholm, Jørgen Steen ; Jungersen, Gregers ; Andersen, Peter ; Follmann, Frank. / A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs. I: Immunology and Cell Biology. 2016 ; Bind 94, Nr. 2. s. 185-195.

Bibtex

@article{3713968e8bcc4d85bfb85cb969f8a87c,
title = "A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs",
abstract = "Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel G{\"o}ttingen minipig animal model, we evaluated the immunogenicity and efficacy of a multi-subunit vaccine formulated in the strong Th1-inducing adjuvant CAF01. We evaluated a mixture of two fusion proteins (Hirep1 and CTH93) designed to promote either neutralizing antibodies or cell-mediated immunity, respectively. Hirep1 is a novel immunogen based on the variant domain (VD) 4 region from major outer membrane protein (MOMP) serovar (Sv) D, SvE and SvF, and CTH93 is a fusion molecule of three antigens (CT043, CT414 and MOMP). Pigs were immunized twice intramuscularly with either Hirep1+CTH93/CAF01, UV-inactivated Chlamydia trachomatis SvD bacteria (UV-SvD/CAF01) or CAF01. The Hirep1+CTH93/CAF01 vaccine induced a strong CMI response against the vaccine antigens and high titers of antibodies, particularly against the VD4 region of MOMP. Sera from Hirep1+CTH93/CAF01 immunized pigs neutralized C. trachomatis SvD and SvF infectivity in vitro. Both Hirep1+CTH93/CAF01 and UV-SvD/CAF01 vaccination protected pigs against a vaginal C. trachomatis SvD infection. In conclusion, the Hirep1+CTH93/CAF01 vaccine proved highly immunogenic and equally protective as UV-SvD/CAF01 showing promise for the development of a subunit vaccine against Chlamydia.",
keywords = "Animals, Antibodies, Neutralizing, Bacterial Outer Membrane Proteins, Bacterial Vaccines, Chlamydia, Chlamydia Infections, Disease Models, Animal, Humans, Immunity, Cellular, Immunization, Interferon-gamma, Recombinant Fusion Proteins, Swine, Swine, Miniature, Th1 Cells, Vaccines, Inactivated, Vaccines, Subunit, Journal Article, Research Support, Non-U.S. Gov't",
author = "B{\o}je, {Sarah Henriette} and Olsen, {Anja Weinreich} and Erneholm, {Karin Susanne} and Agerholm, {J{\o}rgen Steen} and Gregers Jungersen and Peter Andersen and Frank Follmann",
year = "2016",
month = feb,
doi = "10.1038/icb.2015.79",
language = "English",
volume = "94",
pages = "185--195",
journal = "Immunology and Cell Biology",
issn = "0818-9641",
publisher = "nature publishing group",
number = "2",

}

RIS

TY - JOUR

T1 - A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs

AU - Bøje, Sarah Henriette

AU - Olsen, Anja Weinreich

AU - Erneholm, Karin Susanne

AU - Agerholm, Jørgen Steen

AU - Jungersen, Gregers

AU - Andersen, Peter

AU - Follmann, Frank

PY - 2016/2

Y1 - 2016/2

N2 - Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel Göttingen minipig animal model, we evaluated the immunogenicity and efficacy of a multi-subunit vaccine formulated in the strong Th1-inducing adjuvant CAF01. We evaluated a mixture of two fusion proteins (Hirep1 and CTH93) designed to promote either neutralizing antibodies or cell-mediated immunity, respectively. Hirep1 is a novel immunogen based on the variant domain (VD) 4 region from major outer membrane protein (MOMP) serovar (Sv) D, SvE and SvF, and CTH93 is a fusion molecule of three antigens (CT043, CT414 and MOMP). Pigs were immunized twice intramuscularly with either Hirep1+CTH93/CAF01, UV-inactivated Chlamydia trachomatis SvD bacteria (UV-SvD/CAF01) or CAF01. The Hirep1+CTH93/CAF01 vaccine induced a strong CMI response against the vaccine antigens and high titers of antibodies, particularly against the VD4 region of MOMP. Sera from Hirep1+CTH93/CAF01 immunized pigs neutralized C. trachomatis SvD and SvF infectivity in vitro. Both Hirep1+CTH93/CAF01 and UV-SvD/CAF01 vaccination protected pigs against a vaginal C. trachomatis SvD infection. In conclusion, the Hirep1+CTH93/CAF01 vaccine proved highly immunogenic and equally protective as UV-SvD/CAF01 showing promise for the development of a subunit vaccine against Chlamydia.

AB - Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel Göttingen minipig animal model, we evaluated the immunogenicity and efficacy of a multi-subunit vaccine formulated in the strong Th1-inducing adjuvant CAF01. We evaluated a mixture of two fusion proteins (Hirep1 and CTH93) designed to promote either neutralizing antibodies or cell-mediated immunity, respectively. Hirep1 is a novel immunogen based on the variant domain (VD) 4 region from major outer membrane protein (MOMP) serovar (Sv) D, SvE and SvF, and CTH93 is a fusion molecule of three antigens (CT043, CT414 and MOMP). Pigs were immunized twice intramuscularly with either Hirep1+CTH93/CAF01, UV-inactivated Chlamydia trachomatis SvD bacteria (UV-SvD/CAF01) or CAF01. The Hirep1+CTH93/CAF01 vaccine induced a strong CMI response against the vaccine antigens and high titers of antibodies, particularly against the VD4 region of MOMP. Sera from Hirep1+CTH93/CAF01 immunized pigs neutralized C. trachomatis SvD and SvF infectivity in vitro. Both Hirep1+CTH93/CAF01 and UV-SvD/CAF01 vaccination protected pigs against a vaginal C. trachomatis SvD infection. In conclusion, the Hirep1+CTH93/CAF01 vaccine proved highly immunogenic and equally protective as UV-SvD/CAF01 showing promise for the development of a subunit vaccine against Chlamydia.

KW - Animals

KW - Antibodies, Neutralizing

KW - Bacterial Outer Membrane Proteins

KW - Bacterial Vaccines

KW - Chlamydia

KW - Chlamydia Infections

KW - Disease Models, Animal

KW - Humans

KW - Immunity, Cellular

KW - Immunization

KW - Interferon-gamma

KW - Recombinant Fusion Proteins

KW - Swine

KW - Swine, Miniature

KW - Th1 Cells

KW - Vaccines, Inactivated

KW - Vaccines, Subunit

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/icb.2015.79

DO - 10.1038/icb.2015.79

M3 - Journal article

C2 - 26268662

VL - 94

SP - 185

EP - 195

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

SN - 0818-9641

IS - 2

ER -

ID: 171582067