Introduction: Mitral valve area (MVA) planimetry is used to diagnose and classify mitral stenosis (MS) in humans using two-dimensional and three-dimensional echocardiography (MVA_2D and MVA_3D). This study aimed to evaluate agreement, feasibility, and observer variability between MVA_2D and MVA_3D in English Bull Terriers (BT). Our hypotheses were (1) that the MVA of BT is generally smaller than that of breeds with similar body weight and (2) that these techniques could be used to diagnose MS in BTs. Animals: Twenty healthy BTs, 15 healthy Boxers, and 49 BTs with heart disease. Materials and methods: A prospective diagnostic agreement study was conducted. All dogs underwent a thorough clinical examination, conventional transthoracic echocardiography, and three-dimensional echocardiography. Results: Bland–Altman plots (limits of agreement: 0.12–1.5) showed consistent bias and poor agreement between MVA_2D and MVA_3D. For the 69 BTs, MVA_3D (2.1 ± 0.50 cm²) measurements were significantly lower than MVA_2D measurements (2.9 ± 0.60 cm²), and healthy BTs had significantly lower MVA parameters than healthy Boxers (p < 0.001). Intraobserver and interobserver variability were excellent for both MVA_2D and MVA_3D (intraclass correlation coefficient >0.9). Six BTs were diagnosed with MS, with MVA_3D less than 1.8 cm² and a mean transmitral gradient (MTG) of more than 5 mmHg. Conclusions: Both MVA_2D and MVA_3D are feasible, have low observer variability and can be used to diagnose MS in BTs. For assessing the narrowest orifice area, the preferred method is MVA_3D. The smaller MVA in BTs compared to Boxers may indicate some degree of MS.