Tandem mass tag (Tmt) proteomic analysis of saliva in horses with acute abdominal disease

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Alberto Muñoz-Prieto
  • Damián Escribano
  • María Dolores Contreras-Aguilar
  • Anita Horvatić
  • Nicolas Guillemin
  • Jacobsen, Stine
  • José Joaquín Cerón
  • Vladimir Mrljak

The aim of this study was to investigate the changes in the salivary proteome in horses with acute abdominal disease (AAD) using a tandem mass tags (TMT)-based proteomic approach. The saliva samples from eight horses with AAD were compared with six healthy horses in the pro-teomic study. Additionally, saliva samples from eight horses with AAD and eight controls were used to validate lactoferrin (LF) in saliva. The TMT analysis quantified 118 proteins. Of these, 17 differed significantly between horses with AAD and the healthy controls, 11 being downregulated and 6 upregulated. Our results showed the downregulation of gamma-enteric smooth muscle actin (ACTA2), latherin isoform X1, and LF. These proteins could be closely related to an impaired primary immune defense and antimicrobial capacity in the mucosa. In addition, there was an upregu-lation of mucin 19 (MUC19) and the serine protease inhibitor Kazal-type 5 (SPINK5) associated with a protective effect during inflammation. The proteins identified in our study could have the potential to be novel biomarkers for diagnosis or monitoring the physiopathology of the disease, espe-cially LF, which decreased in the saliva of horses with AAD and was successfully measured using a commercially available immunoassay.

Udgave nummer5
StatusUdgivet - 2021

Bibliografisk note

Funding Information:
Funding: AMP was funded by a postdoctoral grant (21239/PD/19. Fundación Séneca, Región de Murcia, Spain). DE has a postdoctoral fellowship granted by the contract “Convocatoria relevo ge-neracional para el fomento de la investigación” by the University of Murcia (Spain), grant number R-1437/2019. DE was also supported by the “Moving Minds CMN” program of the University of Murcia (R-27/2020). MDCA was granted with a predoctoral contract “FPU” of the University of Murcia (R-605/2016). This work was supported by a grant from the Program for Research Groups of Excellence of the Seneca Foundation, Murcia, Spain (grant 19894/GERM/15) and partly supported by the European Structural and Investment Funds (Grant Agreement KK.

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© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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