Tandem mass tag (Tmt) proteomic analysis of saliva in horses with acute abdominal disease
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Tandem mass tag (Tmt) proteomic analysis of saliva in horses with acute abdominal disease. / Muñoz-Prieto, Alberto; Escribano, Damián; Contreras-Aguilar, María Dolores; Horvatić, Anita; Guillemin, Nicolas; Jacobsen, Stine; Cerón, José Joaquín; Mrljak, Vladimir.
I: Animals, Bind 11, Nr. 5, 1304, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Tandem mass tag (Tmt) proteomic analysis of saliva in horses with acute abdominal disease
AU - Muñoz-Prieto, Alberto
AU - Escribano, Damián
AU - Contreras-Aguilar, María Dolores
AU - Horvatić, Anita
AU - Guillemin, Nicolas
AU - Jacobsen, Stine
AU - Cerón, José Joaquín
AU - Mrljak, Vladimir
N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - The aim of this study was to investigate the changes in the salivary proteome in horses with acute abdominal disease (AAD) using a tandem mass tags (TMT)-based proteomic approach. The saliva samples from eight horses with AAD were compared with six healthy horses in the pro-teomic study. Additionally, saliva samples from eight horses with AAD and eight controls were used to validate lactoferrin (LF) in saliva. The TMT analysis quantified 118 proteins. Of these, 17 differed significantly between horses with AAD and the healthy controls, 11 being downregulated and 6 upregulated. Our results showed the downregulation of gamma-enteric smooth muscle actin (ACTA2), latherin isoform X1, and LF. These proteins could be closely related to an impaired primary immune defense and antimicrobial capacity in the mucosa. In addition, there was an upregu-lation of mucin 19 (MUC19) and the serine protease inhibitor Kazal-type 5 (SPINK5) associated with a protective effect during inflammation. The proteins identified in our study could have the potential to be novel biomarkers for diagnosis or monitoring the physiopathology of the disease, espe-cially LF, which decreased in the saliva of horses with AAD and was successfully measured using a commercially available immunoassay.
AB - The aim of this study was to investigate the changes in the salivary proteome in horses with acute abdominal disease (AAD) using a tandem mass tags (TMT)-based proteomic approach. The saliva samples from eight horses with AAD were compared with six healthy horses in the pro-teomic study. Additionally, saliva samples from eight horses with AAD and eight controls were used to validate lactoferrin (LF) in saliva. The TMT analysis quantified 118 proteins. Of these, 17 differed significantly between horses with AAD and the healthy controls, 11 being downregulated and 6 upregulated. Our results showed the downregulation of gamma-enteric smooth muscle actin (ACTA2), latherin isoform X1, and LF. These proteins could be closely related to an impaired primary immune defense and antimicrobial capacity in the mucosa. In addition, there was an upregu-lation of mucin 19 (MUC19) and the serine protease inhibitor Kazal-type 5 (SPINK5) associated with a protective effect during inflammation. The proteins identified in our study could have the potential to be novel biomarkers for diagnosis or monitoring the physiopathology of the disease, espe-cially LF, which decreased in the saliva of horses with AAD and was successfully measured using a commercially available immunoassay.
KW - Acute abdominal disease
KW - Horses
KW - Proteomic
KW - Saliva
KW - Tandem mass tag
U2 - 10.3390/ani11051304
DO - 10.3390/ani11051304
M3 - Journal article
C2 - 33946607
AN - SCOPUS:85108898193
VL - 11
JO - Animals
JF - Animals
SN - 2076-2615
IS - 5
M1 - 1304
ER -
ID: 273641749