Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions. / Jessen, Lisbeth R.; Nielsen, Lise N.; Kieler, Ida N.; Langhorn, Rebecca; Reezigt, Bert J.; Cirera, Susanna.

I: Journal of Veterinary Internal Medicine, Bind 34, Nr. 1, 2020, s. 166-175.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Jessen, LR, Nielsen, LN, Kieler, IN, Langhorn, R, Reezigt, BJ & Cirera, S 2020, 'Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions', Journal of Veterinary Internal Medicine, bind 34, nr. 1, s. 166-175. https://doi.org/10.1111/jvim.15628

APA

Jessen, L. R., Nielsen, L. N., Kieler, I. N., Langhorn, R., Reezigt, B. J., & Cirera, S. (2020). Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions. Journal of Veterinary Internal Medicine, 34(1), 166-175. https://doi.org/10.1111/jvim.15628

Vancouver

Jessen LR, Nielsen LN, Kieler IN, Langhorn R, Reezigt BJ, Cirera S. Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions. Journal of Veterinary Internal Medicine. 2020;34(1):166-175. https://doi.org/10.1111/jvim.15628

Author

Jessen, Lisbeth R. ; Nielsen, Lise N. ; Kieler, Ida N. ; Langhorn, Rebecca ; Reezigt, Bert J. ; Cirera, Susanna. / Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions. I: Journal of Veterinary Internal Medicine. 2020 ; Bind 34, Nr. 1. s. 166-175.

Bibtex

@article{841277c960a54221adcd4bd132862a13,
title = "Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions",
abstract = "Background: Specific biomarkers of pyelonephritis (PN) in cats are lacking. MicroRNAs (miRNAs) have diagnostic potential in human nephropathies. Objectives: To investigate the presence/stability of miRNAs in whole urine of cats and the discriminatory potential of selected urinary miRNAs for PN in cats. Animals: Twelve healthy cats, 5 cats with PN, and 13 cats with chronic kidney disease (n = 5), subclinical bacteriuria (n = 3), and ureteral obstructions (n = 5) recruited from 2 companion animal hospitals. Methods: Prospective case-control study. Expression profiles of 24 miRNAs were performed by quantitative PCR (qPCR). Effect of storage temperature (4°C [24 hours], −20°C, and −80°C) was determined for a subset of miRNAs in healthy cats. Results: Urinary miR-4286, miR-30c, miR-204, miR4454, miR-21, miR-16, miR-191, and miR-30a were detected. For the majority of miRNAs tested, storage at 4°C and −20°C resulted in significantly lower miRNA yield compared to storage at −80°C (mean log2fold changes across miRNAs from −0.5 ± 0.4 SD to −1.20 ± 0.4 SD (4°C versus −80°C) and from −0.7 ± 0.2 SD to −1.20 ± 0.3 SD (−20°C versus −80°C)). Cats with PN had significantly upregulated miR-16 with a mean log2fold change of 1.0 ± 0.4 SD, compared with controls (−0.1 ± 0.2, P =.01) and other urological conditions (0.6 ± 0.3, P =.04). Conclusions: Upregulation of miR16 might be PN-specific, pathogen-specific (Escherichia coli), or both.",
keywords = "Biomarker, CKD, feline, pathogen-directed microRNA expression, subclinical bacteriuria, ureteral obstruction, UTI",
author = "Jessen, {Lisbeth R.} and Nielsen, {Lise N.} and Kieler, {Ida N.} and Rebecca Langhorn and Reezigt, {Bert J.} and Susanna Cirera",
year = "2020",
doi = "10.1111/jvim.15628",
language = "English",
volume = "34",
pages = "166--175",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions

AU - Jessen, Lisbeth R.

AU - Nielsen, Lise N.

AU - Kieler, Ida N.

AU - Langhorn, Rebecca

AU - Reezigt, Bert J.

AU - Cirera, Susanna

PY - 2020

Y1 - 2020

N2 - Background: Specific biomarkers of pyelonephritis (PN) in cats are lacking. MicroRNAs (miRNAs) have diagnostic potential in human nephropathies. Objectives: To investigate the presence/stability of miRNAs in whole urine of cats and the discriminatory potential of selected urinary miRNAs for PN in cats. Animals: Twelve healthy cats, 5 cats with PN, and 13 cats with chronic kidney disease (n = 5), subclinical bacteriuria (n = 3), and ureteral obstructions (n = 5) recruited from 2 companion animal hospitals. Methods: Prospective case-control study. Expression profiles of 24 miRNAs were performed by quantitative PCR (qPCR). Effect of storage temperature (4°C [24 hours], −20°C, and −80°C) was determined for a subset of miRNAs in healthy cats. Results: Urinary miR-4286, miR-30c, miR-204, miR4454, miR-21, miR-16, miR-191, and miR-30a were detected. For the majority of miRNAs tested, storage at 4°C and −20°C resulted in significantly lower miRNA yield compared to storage at −80°C (mean log2fold changes across miRNAs from −0.5 ± 0.4 SD to −1.20 ± 0.4 SD (4°C versus −80°C) and from −0.7 ± 0.2 SD to −1.20 ± 0.3 SD (−20°C versus −80°C)). Cats with PN had significantly upregulated miR-16 with a mean log2fold change of 1.0 ± 0.4 SD, compared with controls (−0.1 ± 0.2, P =.01) and other urological conditions (0.6 ± 0.3, P =.04). Conclusions: Upregulation of miR16 might be PN-specific, pathogen-specific (Escherichia coli), or both.

AB - Background: Specific biomarkers of pyelonephritis (PN) in cats are lacking. MicroRNAs (miRNAs) have diagnostic potential in human nephropathies. Objectives: To investigate the presence/stability of miRNAs in whole urine of cats and the discriminatory potential of selected urinary miRNAs for PN in cats. Animals: Twelve healthy cats, 5 cats with PN, and 13 cats with chronic kidney disease (n = 5), subclinical bacteriuria (n = 3), and ureteral obstructions (n = 5) recruited from 2 companion animal hospitals. Methods: Prospective case-control study. Expression profiles of 24 miRNAs were performed by quantitative PCR (qPCR). Effect of storage temperature (4°C [24 hours], −20°C, and −80°C) was determined for a subset of miRNAs in healthy cats. Results: Urinary miR-4286, miR-30c, miR-204, miR4454, miR-21, miR-16, miR-191, and miR-30a were detected. For the majority of miRNAs tested, storage at 4°C and −20°C resulted in significantly lower miRNA yield compared to storage at −80°C (mean log2fold changes across miRNAs from −0.5 ± 0.4 SD to −1.20 ± 0.4 SD (4°C versus −80°C) and from −0.7 ± 0.2 SD to −1.20 ± 0.3 SD (−20°C versus −80°C)). Cats with PN had significantly upregulated miR-16 with a mean log2fold change of 1.0 ± 0.4 SD, compared with controls (−0.1 ± 0.2, P =.01) and other urological conditions (0.6 ± 0.3, P =.04). Conclusions: Upregulation of miR16 might be PN-specific, pathogen-specific (Escherichia coli), or both.

KW - Biomarker

KW - CKD

KW - feline

KW - pathogen-directed microRNA expression

KW - subclinical bacteriuria

KW - ureteral obstruction

KW - UTI

U2 - 10.1111/jvim.15628

DO - 10.1111/jvim.15628

M3 - Journal article

C2 - 31721298

AN - SCOPUS:85075013615

VL - 34

SP - 166

EP - 175

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 1

ER -

ID: 230792043